Title

Deoxycholateinduces the preferential hydrolysis of polyphosphoinositides by human platelet and rat corneal phospholipase C

Document Type

Article

Date of Publication

6-14-1985

Publication Title

Biochemical and Biophysical Research Communications

First Page

411

Last Page

436

Abstract

Deoxycholate promotes phospholipase C degradation of endogenous phosphatidyl[3H]inositol (PI), phosphatidyl[3H]inositol monophosphate (PIP) and phosphatidyl[3H]inositol bisphosphate (PIP2) in rat cornea and human platelets. Hydrolysis of phosphatidyl[3H]inositol significantly lags polyphospho[3H]inositide degradation. Concomitantly, formation of [3H]inositol monophosphate (IP1) lags behind [3H]inositol bisphosphate (IP2) and [3H]inositol trisphosphate (IP3) production. These results demonstrate that rat cornea and human platelet phospholipase C cause a preferential hydrolysis of the endogenous polyphosphoinositides rather than phosphatidylinositol.

DOI

https://doi.org/10.1016/0006-291X(85)90166-4

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