Title

Amperometric detection of methanol with a methanol dehydrogenase modified electrode sensor

Document Type

Article

Date of Publication

3-15-2007

Publication Title

Journal of Electroanalytical Chemistry

First Page

125

Last Page

131

Location

Department of Chemistry, The University of Toledo, Toledo, OH

Abstract

An amperometric enzyme electrode was developed by immobilizing the quinoprotein methanol dehydrogenase from Methylobacterium extorquens AM1 onto a glassy carbon electrode. Ferrocene (FC), ferrocene carboxylic acid, N,N,N′,N′-tetramethyl-1,4-phenylenediamine (TMPD), N,N-dimethyl-p-phenylenediamine (DMPD), phenazine methosulfate (PMeS) and Wurster blue (WB) were evaluated as electron-transfer mediators. DMPD, TMPD and Fc were found to be effective electron-transfer mediators for the enzyme with only ferrocene exhibiting a stable response in the presence of oxygen. The best response for the detection of methanol was achieved with a 100 μM suspension of ferrocene in 100 mM Tris/HCl buffer pH 9.0. The sensor utilized very small quantities of enzyme, and exhibited excellent reproducibility and stability with a detection limit of 0.5 μM (S/N = 3) and a linear range of 0.5–200 μM.

DOI

https://doi.org/10.1016/j.jelechem.2006.10.039

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