Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain: Comparative roles of LIN28A and C19MC

F. A. Jakobiec; M. Kool; A. M. Stagner; S. M. Pfister; R. C. Eagle; Alan Proia; A. Korshunov

doi:10.1016/j.ajo.2015.03.002

Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain: Comparative roles of LIN28A and C19MC

F. A. Jakobiec, M. Kool, A. M. Stagner, S. M. Pfister, R. C. Eagle, Alan Proia, A. Korshunov

Campbell University

Research output: Contribution to journal › Article › peer-review

Abstract

<p> <h3 id="x-x-sectitle0010"> Purpose </h3> <p id="x-x-abspara0010"> To compare immunohistochemical and genetic overlaps and differences between intraocular <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/neuroepithelioma" title="Learn more about Neuroepithelioma from ScienceDirect's AI-generated Topic Pages"> medulloepitheliomas </a> and embryonal tumors with multilayered <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/rosette-formation" title="Learn more about Rosette Formation from ScienceDirect's AI-generated Topic Pages"> rosettes </a> of the brain. <h3 id="x-x-sectitle0015"> Design </h3> <p id="x-x-abspara0015"> Retrospective histopathologic, immunohistochemical, and <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/genetic-analysis" title="Learn more about Genetic Analysis from ScienceDirect's AI-generated Topic Pages"> genetic analysis </a> of 20 intraocular medulloepitheliomas. <h3 id="x-x-sectitle0020"> Methods </h3> <p id="x-x-abspara0020"> (1) Review of clinical data and hematoxylin-eosin-stained sections with (2) immunohistochemical staining of <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/paraffin" title="Learn more about Paraffin from ScienceDirect's AI-generated Topic Pages"> paraffin sections </a> using a <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/polyclonal-antibody" title="Learn more about Polyclonal Antibody from ScienceDirect's AI-generated Topic Pages"> polyclonal antibody </a> against the protein LIN28A, and (3) fluorescence in situ hybridization (FISH) testing for the amplification of the <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/gene-locus" title="Learn more about Gene Locus from ScienceDirect's AI-generated Topic Pages"> genetic locus </a> 19q13.42 involving the <em> C19MC </em> cluster of <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/microrna" title="Learn more about microRNA from ScienceDirect's AI-generated Topic Pages"> miRNA </a> . Ten <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/retinoblastoma" title="Learn more about Retinoblastoma from ScienceDirect's AI-generated Topic Pages"> retinoblastomas </a> served as controls and to determine the specificity of these biomarkers for intraocular medulloepitheliomas. <h3 id="x-x-sectitle0025"> Results </h3> <p id="x-x-abspara0025"> Nineteen of the 20 intraocular medulloepitheliomas were either diffusely or focally LIN28A positive (weak, moderate, or strong). The most intense positivity correlated with aggressive behavior such as intraocular tissue invasion or extraocular extension. None of the cases studied by FISH harbored an <a href="https://www.sciencedirect.com/topics/medicine-and-dentistry/amplicon" title="Learn more about Amplicon from ScienceDirect's AI-generated Topic Pages"> amplicon </a> for <em> C19MC </em> . The 10 retinoblastomas were LIN28A and <em> C19MC </em> negative. <h3 id="x-x-sectitle0030"> Conclusion </h3> <p id="x-x-abspara0030"> LIN28A has a putative role in oncogenesis and is found only in embryonic cells and malignancies. Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain both display LIN28A positivity. Only the latter, however, display amplification of the 19q13.42 locus involving <em> C19MC </em> , implying that other causative factors are at play in intraocular medulloepitheliomas. More aggressive tumor behavior within the eye can be partially predicted by LIN28A staining intensity. </p> </p> </p> </p> </p></p>

Original language	American English
Journal	American Journal of Ophthalmology
Volume	159
DOIs	https://doi.org/10.1016/j.ajo.2015.03.002
State	Published - Mar 5 2015

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Osteopathic Medicine and Osteopathy

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https://doi.org/10.1016/j.ajo.2015.03.002

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@article{cc2ba38744714477a7ffd8b6864b6a02,

title = "Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain: Comparative roles of LIN28A and C19MC",

abstract = " Purpose To compare immunohistochemical and genetic overlaps and differences between intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain. Design Retrospective histopathologic, immunohistochemical, and genetic analysis of 20 intraocular medulloepitheliomas. Methods (1) Review of clinical data and hematoxylin-eosin-stained sections with (2) immunohistochemical staining of paraffin sections using a polyclonal antibody against the protein LIN28A, and (3) fluorescence in situ hybridization (FISH) testing for the amplification of the genetic locus 19q13.42 involving the C19MC cluster of miRNA . Ten retinoblastomas served as controls and to determine the specificity of these biomarkers for intraocular medulloepitheliomas. Results Nineteen of the 20 intraocular medulloepitheliomas were either diffusely or focally LIN28A positive (weak, moderate, or strong). The most intense positivity correlated with aggressive behavior such as intraocular tissue invasion or extraocular extension. None of the cases studied by FISH harbored an amplicon for C19MC . The 10 retinoblastomas were LIN28A and C19MC negative. Conclusion LIN28A has a putative role in oncogenesis and is found only in embryonic cells and malignancies. Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain both display LIN28A positivity. Only the latter, however, display amplification of the 19q13.42 locus involving C19MC , implying that other causative factors are at play in intraocular medulloepitheliomas. More aggressive tumor behavior within the eye can be partially predicted by LIN28A staining intensity. ",

author = "Jakobiec, {F. A.} and M. Kool and Stagner, {A. M.} and Pfister, {S. M.} and Eagle, {R. C.} and Alan Proia and A. Korshunov",

year = "2015",

month = mar,

day = "5",

doi = "10.1016/j.ajo.2015.03.002",

language = "American English",

volume = "159",

journal = "American Journal of Ophthalmology",

}

TY - JOUR

T1 - Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain: Comparative roles of LIN28A and C19MC

AU - Jakobiec, F. A.

AU - Kool, M.

AU - Stagner, A. M.

AU - Pfister, S. M.

AU - Eagle, R. C.

AU - Proia, Alan

AU - Korshunov, A.

PY - 2015/3/5

Y1 - 2015/3/5

N2 - Purpose To compare immunohistochemical and genetic overlaps and differences between intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain. Design Retrospective histopathologic, immunohistochemical, and genetic analysis of 20 intraocular medulloepitheliomas. Methods (1) Review of clinical data and hematoxylin-eosin-stained sections with (2) immunohistochemical staining of paraffin sections using a polyclonal antibody against the protein LIN28A, and (3) fluorescence in situ hybridization (FISH) testing for the amplification of the genetic locus 19q13.42 involving the C19MC cluster of miRNA . Ten retinoblastomas served as controls and to determine the specificity of these biomarkers for intraocular medulloepitheliomas. Results Nineteen of the 20 intraocular medulloepitheliomas were either diffusely or focally LIN28A positive (weak, moderate, or strong). The most intense positivity correlated with aggressive behavior such as intraocular tissue invasion or extraocular extension. None of the cases studied by FISH harbored an amplicon for C19MC . The 10 retinoblastomas were LIN28A and C19MC negative. Conclusion LIN28A has a putative role in oncogenesis and is found only in embryonic cells and malignancies. Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain both display LIN28A positivity. Only the latter, however, display amplification of the 19q13.42 locus involving C19MC , implying that other causative factors are at play in intraocular medulloepitheliomas. More aggressive tumor behavior within the eye can be partially predicted by LIN28A staining intensity.

AB - Purpose To compare immunohistochemical and genetic overlaps and differences between intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain. Design Retrospective histopathologic, immunohistochemical, and genetic analysis of 20 intraocular medulloepitheliomas. Methods (1) Review of clinical data and hematoxylin-eosin-stained sections with (2) immunohistochemical staining of paraffin sections using a polyclonal antibody against the protein LIN28A, and (3) fluorescence in situ hybridization (FISH) testing for the amplification of the genetic locus 19q13.42 involving the C19MC cluster of miRNA . Ten retinoblastomas served as controls and to determine the specificity of these biomarkers for intraocular medulloepitheliomas. Results Nineteen of the 20 intraocular medulloepitheliomas were either diffusely or focally LIN28A positive (weak, moderate, or strong). The most intense positivity correlated with aggressive behavior such as intraocular tissue invasion or extraocular extension. None of the cases studied by FISH harbored an amplicon for C19MC . The 10 retinoblastomas were LIN28A and C19MC negative. Conclusion LIN28A has a putative role in oncogenesis and is found only in embryonic cells and malignancies. Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain both display LIN28A positivity. Only the latter, however, display amplification of the 19q13.42 locus involving C19MC , implying that other causative factors are at play in intraocular medulloepitheliomas. More aggressive tumor behavior within the eye can be partially predicted by LIN28A staining intensity.

UR - https://cufind.campbell.edu/medicine_school/451

UR - https://doi.org/10.1016/j.ajo.2015.03.002

U2 - 10.1016/j.ajo.2015.03.002

DO - 10.1016/j.ajo.2015.03.002

M3 - Article

VL - 159

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

ER -

Intraocular medulloepitheliomas and embryonal tumors with multilayered rosettes of the brain: Comparative roles of LIN28A and C19MC

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