TY - JOUR
T1 - Amperometric detection of methanol with a methanol dehydrogenase modified electrode sensor
AU - Liu, Qinfeng
AU - Kirchhoff, J. R.
PY - 2007/3/15
Y1 - 2007/3/15
N2 - An amperometric enzyme electrode was developed by immobilizing the quinoprotein methanol dehydrogenase from Methylobacterium extorquens AM1 onto a glassy carbon electrode. Ferrocene (FC), ferrocene carboxylic acid , N , N , N ′, N ′-tetramethyl-1,4-phenylenediamine (TMPD), N , N -dimethyl- p -phenylenediamine (DMPD), phenazine methosulfate (PMeS) and Wurster blue (WB) were evaluated as electron-transfer mediators. DMPD, TMPD and Fc were found to be effective electron-transfer mediators for the enzyme with only ferrocene exhibiting a stable response in the presence of oxygen. The best response for the detection of methanol was achieved with a 100 μM suspension of ferrocene in 100 mM Tris/HCl buffer pH 9.0. The sensor utilized very small quantities of enzyme, and exhibited excellent reproducibility and stability with a detection limit of 0.5 μM ( S / N = 3) and a linear range of 0.5–200 μM.
AB - An amperometric enzyme electrode was developed by immobilizing the quinoprotein methanol dehydrogenase from Methylobacterium extorquens AM1 onto a glassy carbon electrode. Ferrocene (FC), ferrocene carboxylic acid , N , N , N ′, N ′-tetramethyl-1,4-phenylenediamine (TMPD), N , N -dimethyl- p -phenylenediamine (DMPD), phenazine methosulfate (PMeS) and Wurster blue (WB) were evaluated as electron-transfer mediators. DMPD, TMPD and Fc were found to be effective electron-transfer mediators for the enzyme with only ferrocene exhibiting a stable response in the presence of oxygen. The best response for the detection of methanol was achieved with a 100 μM suspension of ferrocene in 100 mM Tris/HCl buffer pH 9.0. The sensor utilized very small quantities of enzyme, and exhibited excellent reproducibility and stability with a detection limit of 0.5 μM ( S / N = 3) and a linear range of 0.5–200 μM.
UR - https://cufind.campbell.edu/pharmacy/1969
UR - https://doi.org/10.1016/j.jelechem.2006.10.039
U2 - 10.1016/j.jelechem.2006.10.039
DO - 10.1016/j.jelechem.2006.10.039
M3 - Article
VL - 601
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
ER -